Chemogenetic approaches using Designer Receptors Exclusively Activated by Designer Drugs (DREADD, a family of engineered GPCRs) were recently employed in microglia. Here, we used Cx3cr1:R26 mice to express Gi-DREADD (hM4Di) on CX3CR1 cells, comprising microglia and some peripheral immune cells, and found that activation of hM4Di on long-lived CX3CR1 cells induced hypolocomotion. Unexpectedly, Gi-DREADD-induced hypolocomotion was preserved when microglia were depleted. Consistently, specific activation of microglial hM4Di cannot induce hypolocomotion in Tmem119:R26 mice. Flow cytometric and histological analysis showed hM4Di expression in peripheral immune cells, which may be responsible for the hypolocomotion. Nevertheless, depletion of splenic macrophages, hepatic macrophages, or CD4 T cells did not affect Gi-DREADD-induced hypolocomotion. Our study demonstrates that rigorous data analysis and interpretation are needed when using Cx3cr1 mouse line to manipulate microglia.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10906107PMC
http://dx.doi.org/10.1038/s41380-023-02128-6DOI Listing

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