Antibacterial and Anticancer Activity, Acute Toxicity, and Solubility of Co-crystals of 5-Fluorouracil and Trimethoprim.

5-Fluorouracil is mainly used for the treatment of tumors and has relatively high toxicity. Trimethoprim is a common broad-spectrum antibiotic agent with extremely poor water solubility. We hoped to solve these problems by synthesizing co-crystals (compound ) of 5-fluorouracil and trimethoprim. Solubility tests showed that the solubility of compound was improved compared to that of trimethoprim. In vitro anticancer activity tests of compound showed higher activity against human breast cancer cells than 5-fluorouracil. Acute toxicity showed that its toxicity was much lower than that of 5-fluorouracil. In the test of anti- activity, compound showed much stronger antibacterial activity than trimethoprim.