Plastid DNA Barcoding and cDNA Fragment Isolation of : A Promising Bioresource for Biodiesel Production.

belonging to the family is currently used for biodiesel production, and rapid development in plant-based biofuel production has led to its increasing demand. However, massive utilization of bio-industrial plants has led to conservation issues. Moreover, genetic information on is still limited, which is crucial for developmental, physiological, and molecular studies. Studying gene expression is essential to explain plant physiological processes. Nonetheless, this technique requires sensitive and precise measurement of messenger RNA (mRNA). In addition, the presence of internal control genes is important to avoid bias. Therefore, collecting and preserving genetic data for is indispensable. In this study, we aimed to evaluate the application of plastid loci, , and , to the DNA barcode of for use in conservation programs. In addition, we isolated and cloned the () gene fragment for use in gene expression studies. Sequence information was analyzed by comparison with other plants. For actin fragment isolation, reverse-transcription polymerase chain reaction was used. Molecular cloning of was performed using the pTA2 plasmid before sequencing. We successfully isolated and cloned 592 and 840 bp of and fragment genes, respectively. The barcoding marker, rather than the plastidial marker, provided discriminative molecular phylogenetic data for . We also isolated 986 bp of gene fragments. Our phylogenetic analysis demonstrated that is closely related to the gene (97% identity). Our results suggest that could be further developed and used as a barcoding marker for . Moreover, the gene could be further investigated for use in gene expression studies of plant.