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Phage-Inducible Chromosomal Islands as a Diagnostic Platform to Capture and Detect Bacterial Pathogens. | LitMetric

Phage-Inducible Chromosomal Islands as a Diagnostic Platform to Capture and Detect Bacterial Pathogens.

Adv Sci (Weinh)

Division of Biomedical Engineering, James Watt School of Engineering, University of Glasgow, Glasgow, G12 8QQ, UK.

Published: August 2023

AI Article Synopsis

  • - Phage-inducible chromosomal islands (PICIs) are specialized genetic elements that use virus components to help them move and spread, and they are now being explored as innovative antibacterial tools in biotechnology.
  • - The authors developed a user-friendly diagnostic system utilizing PICI transduction to selectively isolate and detect harmful bacteria like Escherichia coli and Staphylococcus aureus, achieving high sensitivity with detection limits as low as ≈50 CFU/mL.
  • - Unlike traditional methods that can't tell between live and dead bacteria, this new system allows for the visual detection of only living pathogens by using a reporter gene within the PICI, advancing the field of synthetic biology and biosensing.

Article Abstract

Phage-inducible chromosomal islands (PICIs) are a family of phage satellites that hijack phage components to facilitate their mobility and spread. Recently, these genetic constructs are repurposed as antibacterial drones, enabling a new toolbox for unorthodox applications in biotechnology. To illustrate a new suite of functions, the authors have developed a user-friendly diagnostic system, based upon PICI transduction to selectively enrich bacteria, allowing the detection and sequential recovery of Escherichia coli and Staphylococcus aureus. The system enables high transfer rates and sensitivities in comparison with phages, with detection down to ≈50 CFU mL . In contrast to conventional detection strategies, which often rely on nucleic acid molecular assays, and cannot differentiate between dead and live organisms, this approach enables visual sensing of viable pathogens only, through the expression of a reporter gene encoded in the PICI. The approach extends diagnostic sensing mechanisms beyond cell-free synthetic biology strategies, enabling new synthetic biology/biosensing toolkits.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10460865PMC
http://dx.doi.org/10.1002/advs.202301643DOI Listing

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