[Analysis of Gene Recombination between and -, and - Loci].

Objective: To investigate the recombinations within the human leukocyte antigen (HLA) region in two families.

Methods: Genomic DNA was extracted from the peripheral blood specimens of the different family members. and - loci were genotyped using polymerase chain reaction-sequence specific oligonucleotide probing technique (PCR-SSO) and next-generation sequencing technique. HLA haplotype was determined by genetic analysis of the pedigree.

Results: The haplotypes of and in the family 1 were recombined between and loci, which formed the haplotype of The haplotypes of and in the family 2 were recombined between and loci, which formed the haplotype of .

Conclusion: The gene recombination events between and - and - loci were found respectively in two Chinese Han families.