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Complete genome sequence of Streptomyces sp. HNA39, a new cyclizidine producer isolated from a South China Sea sediment. | LitMetric

AI Article Synopsis

  • Streptomyces sp. HNA39, sourced from coastal sediments, is a candidate for producing anticancer compounds, identified from a collection of 448 actinomycetes.
  • Its complete genome consists of over 7.3 million base pairs and contains 29 biosynthetic gene clusters (BGCs) associated with secondary metabolites, including one resembling the cyclizidine BGC of another Streptomyces species.
  • The organism also produces bafilomycins and the cyclization process for its unique cyclopropane structure is still unknown, with further research needed on the genomic data to clarify this mechanism.

Article Abstract

Streptomyces sp. HNA39 is a promising candidate for the production of antineoplastic metabolites screened from a collection of 448 actinomycetes derived from coastal sediments. The complete genome sequence of HNA39 comprises a 7,351,753-bp linear chromosome with a GC content of 71.94%. Whole genome analysis reveals the presence of 29 putative biosynthetic gene clusters (BGCs) encoding secondary metabolites. Among them, a type I PKS BGC shows an 82% similarity with the cyclizidine (CLD) BGC identified from Streptomyces NCIB 11649. LC-MS profiles further supported the production of new CLD congeners. Bafilomycins were also found produced in abundance, corresponding to another type I PKS BGC highly homologous to that of bafilomycin B1 from S. lohii. CLDs are indolizidine alkaloids consisting a fused five- and six-membered ring system with an intriguing cyclopropane terminal linked by a trans-dienic chain. The cyclization mechanism of the cylopropyl ring, one of its pharmacophores, is still unknown. Genome sequencing of the new CLD producer and subsequent comparative analysis of their gene clusters would further our understanding of the chemistry behind cyclopropane formation.

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Source
http://dx.doi.org/10.1016/j.margen.2023.101033DOI Listing

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