AI Article Synopsis
- Multi-color fluorescence imaging helps researchers study interactions between cell structures, but issues like mechanical drift and chromatic aberration can reduce image quality.
- The authors introduce OFM Corrector, an extension of the Scipion framework that corrects these distortions in real-time using advanced registration techniques.
- Their software, which is user-friendly and freely available, offers a practical solution for improving image resolution in various biological research applications.
Article Abstract
Multi-color fluorescence imaging is a powerful tool for studying the spatial relationships and interactions among sub-cellular structures in biological specimens. However, if improperly corrected, geometrical distortions caused by mechanical drift, refractive index mismatch, or chromatic aberration can lead to lower image resolution. In this paper, we present an extension of the image processing framework of Scipion by integrating a protocol called OFM Corrector, which corrects geometrical distortions in real-time using a B-spline-based elastic continuous registration technique. Our proposal provides a simple strategy to overcome chromatic aberration by digitally re-aligning color channels in multi-color fluorescence microscopy images, even in 3D or time. Our method relies on a geometrical calibration, which we do with fluorescent beads excited by different wavelengths of light and subsequently registered to get the elastic warp as a reference to correct chromatic shift. Our software is freely available with a user-friendly GUI and can be broadly used for various biological imaging problems. The paper presents a valuable tool for researchers working in light microscopy facilities.
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| http://dx.doi.org/10.1088/2050-6120/ace153 | DOI Listing |
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