AI Article Synopsis
- The study focuses on lncRNA PTOV1-AS1's role in hepatocellular carcinoma (HCC) and its contribution to resistance against the cancer drug sorafenib.
- Higher levels of PTOV1-AS1 were found in HCC tissues compared to normal tissues, and its expression correlates with patient survival rates.
- Knocking down PTOV1-AS1 reduced cell growth and invasion in sorafenib-resistant HCC cells by interacting with miR-505, suggesting that targeting PTOV1-AS1 could improve treatment outcomes.
Article Abstract
Increasing evidence has displayed the vital influence of lncRNA in tumorigenesis and chemoresistance of cancer treatment. This study investigated the function of lncRNA PTOV1-AS1 in hepatocellular carcinoma (HCC) and its role in sorafenib resistance. The relative expression of lncRNA and miRNA was measured by RT-qPCR. The cellular activities including cell proliferation and invasion were explored by CCK-8 and Transwell assays. Bioinformatics analysis and dual-luciferase reporter assay were used to predict the targeting miRNA of PTOV1-AS1. The expression levels of PTOV1-AS1 were higher in HCC tissues than that in the normal tissues and associated with patients' overall survival. Knockdown of PTOV1-AS1 decreased cell proliferation rate and invasion number. After treatment with different concentrations of sorafenib, the sorafenib-resistant hepatoma cells were conducted. PTOV1-AS1 expression levels were increased in HepG2-SR and Huh7-SR cells. PTOV1-AS1 knockdown repressed the proliferation, invasion, and drug resistance of sorafenib-resistant HCC cells by targeting the expression of miR-505. In conclusion, the expression of PTOV1-AS1 is increased in HCC and sorafenib-resistance HCC cells, as well as is associated with patients' prognosis. Inhibition of PTOV1-AS1 expression can reduce the resistance of sorafenib-resistant HCC cells, which may play a role by targeting the negative regulation of miR-505 expression.
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| http://dx.doi.org/10.1002/jbt.23437 | DOI Listing |
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