Under certain culture conditions, naive human pluripotent stem cells can generate human blastocyst-like structures (called human blastoids). Human blastoids serve as an accessible model for human blastocysts and are amenable for large-scale production. Here, we describe a detailed step-by-step protocol for the robust and high-efficient generation of human blastoids from naive human pluripotent stem cells.
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Article Synopsis
- * Research on these models, including comparisons across species such as mice, humans, and ungulates (pigs and cattle), enhances our knowledge of both unique and shared developmental mechanisms.
- * The review highlights recent progress in stem cell technology and synthetic embryo models, emphasizing their role in advancing regenerative medicine and improving our understanding of species-specific developmental biology.
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