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Monitoring molting in a conventional luminometer. | LitMetric

Monitoring molting in a conventional luminometer.

MethodsX

Department of Biochemistry and Genetics, School of Sciences, University of Navarra, Calle Irunlarrea 1, 31008, Pamplona, Spain.

Published: June 2023

Molting is an essential developmental process in However, the study of molting in the worm has been limited by the lack of automated techniques that allow monitoring the process in a simple way. In 2015, Olmedo . published an automated method to monitor the timing of each larval stage and molt in using bioluminescence. This new method has greatly contributed to the study of molting in this organism but requires the use of a high-sensitivity luminometer, which many laboratories do not have. We have adapted the method to a conventional luminometer, so that it can be used by most laboratories that work with and do not have high-sensitivity equipment.•A customization of a method to study molting in using a conventional luminometer instead of a high-sensitivity one.•This adaptation allows most laboratories to use their routine luminometers to study molting in .•Although the use of a high-sensitivity luminometer, as proposed by Olmedo ., remains the gold standard for studying molting, this adaptation is suitable for studying significant differences in molting and the duration of larval stages between different strains of .

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10279910PMC
http://dx.doi.org/10.1016/j.mex.2023.102235DOI Listing

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