Nuclear magnetic resonance (NMR) experiments utilizing parahydrogen-induced polarization (PHIP) were performed to elucidate the PHIP activity of the synthetic 236 kDa biopolymer poly-γ-(4-propargyloxy)-benzyl-L-glutamate) (PPOBLG). The homopolypeptide was successfully hyperpolarized and the enhanced signals were detected in 11.7 T solution NMR as a function of the PPOBLG concentration. The hydrogenation with parahydrogen caused signal enhancements of 800 and more for the vinyl protons of the side chain at low substrate concentration. As a result of this high enhancement factor, even at 13 nM of PPOBLG, a single scan H-NMR detection of the hyperpolarized protons was possible, owing to the combination of hyperpolarization and density of PHIP active sites.
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| http://dx.doi.org/10.1038/s41598-023-37202-0 | DOI Listing |
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