EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral purification, titering, and multiple techniques to analyze infection efficacy. This protocol produces high infectivity in C57BL/6 mice which will aid investigators in generating preclinical data.
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| http://dx.doi.org/10.1016/j.xpro.2023.102368 | DOI Listing |
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Identification of EcoHIV-Infected Cells in Microglia-Manipulated Transgenic Mice.
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Department of Pharmacology and Physiology, Drexel University College of Medicine, USA. Electronic address:
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- * Chemogenetic activation of NAc astrocytes showed potential in reversing the effects of EcoHIV on cocaine sensitization, suggesting that targeting these astrocytes could offer strategies for managing cocaine-related behaviors in PLWH.
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View Article and Find Full Text PDFA reproducible murine model of studying HIV-associated brain damage in stroke.
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January 2025
Department of Anatomy and Neurobiology, College of Medicine, the University of Tennessee Health Science Center, Memphis, TN 38163, USA; Neuroscience Institute, the University of Tennessee Health Science Center, Memphis, TN 38163, USA. Electronic address:
Background: Emerging clinical and epidemiological data indicates that human immunodeficiency virus (HIV) is associated with an increased risk of stroke and aggravated brain damage. We aimed to develop a reproducible murine model of photothrombotic-stroke with HIV infection that mimics the clinical situation.
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