Bioremediation of Catechol and Concurrent Accumulation of Biocompounds by the Microalga .

Burgeoning commercial applications of catechol have led to its excessive accumulation in the environment, thereby posing a severe ecological threat. Bioremediation has emerged as a promising solution. The potential of the microalga to degrade catechol and use the byproduct as a carbon source was investigated in this study. Catechol significantly increased growth and was rapidly catabolized within 60 h of cultivation. Transcriptomic analysis highlighted the key genes involved in catechol degradation. Real-time polymerase chain reaction (RT-PCR) analysis showed that transcription of key genes , and involved in the ortho-cleavage pathway was remarkably increased by 2.9-, 4.2-, and 2.4- fold, respectively. Key primary metabolite content was also markedly altered, with a specific increment in polyunsaturated fatty acids. Electron microscopy and antioxidant analysis showed that could tolerate catechol treatment without morphological aberrations or oxidative stress. The findings provide a strategy for in the bioremediation of catechol and concurrent polyunsaturated fatty acids (PUFA) accumulation.