Tomato is cultivated worldwide as a nutrient-rich vegetable crop. Tomato wilt disease caused by Fusarium oxysporum f.sp. Lycopersici (Fol) is one of the most serious fungal diseases posing threats to tomato production. Recently, the development of Spray-Induced Gene Silencing (SIGS) directs a novel plant disease management by generating an efficient and environmental friendly biocontrol agent. Here, we characterized that FolRDR1 (RNA-dependent RNA polymerase 1) mediated the pathogen invasion to the host plant tomato, and played as an essential regulator in pathogen development and pathogenicity. Our fluorescence tracing data further presented that effective uptakes of FolRDR1-dsRNAs were observed in both Fol and tomato tissues. Subsequently, exogenous application of FolRDR1-dsRNAs on pre-Fol-infected tomato leaves resulted in significant alleviation of tomato wilt disease symptoms. Particularly, FolRDR1-RNAi was highly specific without sequence off-target in related plants. Our results of pathogen gene-targeting RNAi have provided a new strategy for tomato wilt disease management by developing an environmentally-friendly biocontrol agent.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10313012 | PMC |
| http://dx.doi.org/10.1371/journal.ppat.1011463 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
trans-2-Octenal controls Fusarium oxysporum f. sp. lycopersici, the causal agent of tomato wilt in vitro, in soil and in the field.
Pest Manag Sci
January 2025
Department of Plant Pathology and Weed Research, ARO-the Volcani Institute, Rishon LeZion, Israel.
Background: Fungal plant diseases cause major crop losses. Phytopathogenic fungi's ability to evolve resistance to fungicides, alongside ongoing prohibition of such agents by the European Commission because of their pronounced adverse effects on human health and the environment, make their control a challenge. Moreover, the development of less perilous fungicides is a complex task.
View Article and Find Full Text PDFComparative genomic analysis of Fusarium oxysporum f. sp. lycopersici reveals telomeric duplications of a lineage-specific region carrying SIX8 and PSL1 and genome-wide expansion of Foxy transposable elements.
Int J Biol Macromol
January 2025
State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, China; Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Ministry of Education of China-Hebei Province Joint Innovation Center for Efficient Green Vegetable Industry, College of Horticulture, Hebei Agricultural University, Baoding 071000, China; Division of Plant Sciences, Research School of Biology, Australian National University, Canberra, ACT 2601, Australia. Electronic address:
Fusarium oxysporum f. sp. lycopersici (Fol), the causal agent of tomato wilt disease, is a soil-borne, vascular-colonizing fungal pathogen that severely impacts tomato production in most growing regions worldwide.
View Article and Find Full Text PDFUnderstanding the Impact of Salt Stress on Plant Pathogens Through Phenotypic and Transcriptomic Analysis.
Plants (Basel)
January 2025
Department of Integrated Biological Science, Pusan National University, Busan 46241, Republic of Korea.
For plant diseases to become established, plant pathogens require not only virulence factors and susceptible hosts, but also optimal environmental conditions. The accumulation of high soil salinity can have serious impacts on agro-biological ecosystems. However, the interactions between plant pathogens and salinity have not been fully characterized.
View Article and Find Full Text PDFMarker-assisted selection in segregating populations of tomatoes for resistance to TYLCV, ToMV, and Fusarium wilt.
Mol Biol Rep
January 2025
Department of Agronomy and Plant Breeding Sciences, Agricultural College of Aburaihan, University of Tehran, Pakdasht, Iran.
Background: Tomato yellow leaf curl virus (TYLCV), tomato mosaic virus (ToMV), and Fusarium wilt are three of tomatoes' most important viral and fungal diseases.
Methods And Results: In this study, the application of molecular markers associated with tomato yellow leaf curl virus resistance gene (Ty1), tomato mosaic virus resistance gene (Tm2), and Fusarium wilt resistance gene (I-1) (linked marker) were evaluated. In order to optimize and use SNP markers (by HRM diagnostic method) and SCAR markers, segregating populations of tomatoes were produced by self-pollination of commercial hybrid cultivars.
Metagenomic Sequencing of Tomato Plants with Wilt Symptoms Allows for Strain-Level Pathogen Identification and Genome-Based Characterization.
Phytopathology
January 2025
Virginia Polytechnic Institute and State University, School of Plant and Environmental Science, Blacksburg, Virginia, United States;
Article Synopsis
- Diseases affecting the vascular system in plants can lead to significant economic losses due to rapid destruction of crops, making quick identification of pathogens crucial for effective management.
- The study utilized culture-independent long-read metagenomic sequencing on DNA from tomato plants displaying wilt symptoms to successfully identify pathogenic strains and predict their virulence and resistance traits.
- The research underscores the potential for metagenomic sequencing to become a standard diagnostic tool in plant disease clinics, as the entire analysis can be completed in just two days.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!