Chromosome banding based on base-specific fluorochromes, mainly double staining with chromomycin A (CMA) and 4'-6-diamidino-2-phenylindole (DAPI), has been widely used since the 1970s. This technique allows the differential staining of distinct types of heterochromatin. Afterward, the fluorochromes can be easily removed and leave the preparation ready for sequential procedures such as FISH or immunodetection. Interpretations of similar bands obtained with different techniques, however, merit certain caution. Here we present a detailed protocol for CMA/DAPI staining optimized for plant cytogenetics and call attention to the most common sources of misinterpretation of DAPI bands.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-3226-0_12 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
CMA/DAPI Banding of Plant Chromosomes.
Methods Mol Biol
June 2023
Laboratório de Citogenética e Evolução Vegetal, Departamento de Botânica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
Chromosome banding based on base-specific fluorochromes, mainly double staining with chromomycin A (CMA) and 4'-6-diamidino-2-phenylindole (DAPI), has been widely used since the 1970s. This technique allows the differential staining of distinct types of heterochromatin. Afterward, the fluorochromes can be easily removed and leave the preparation ready for sequential procedures such as FISH or immunodetection.
View Article and Find Full Text PDFCytogenetic characterization and karyotype evolution in six species (Leguminosae).
Genome
July 2023
Laboratório de Citogenética e Evolução Vegetal, Departamento de Botânica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, PE 50670-901 Brasil.
(Benth.) Urb. is a neotropical legume genus from the subtribe Phaseolinae.
View Article and Find Full Text PDFKaryotyping of commercial cultivars of melon (Cucumis melo L.).
Mol Biol Rep
November 2022
Departamento de Ciências Biológicas, Laboratório de Melhoramento de Plantas, Universidade Estadual de Santa Cruz (UESC), Rod. Jorge Amado, km 16, 45662-900, Ilhéus, Brasil.
Background: This study on cultivars of melon (Cucumis melo L.) marketed in Brazil was conducted to obtain information to be used in breeding programs of this species. Little is known about the karyotype variability among C.
View Article and Find Full Text PDFComparative cytogenetics on eight Malagasy Mantellinae (Anura, Mantellidae) and a synthesis of the karyological data on the subfamily.
Comp Cytogenet
February 2022
CIBIO Research Centre in Biodiversity and Genetic Resources, InBIO, Universidade do Porto, Campus Agrário de Vairão, Rua Padre Armando Quintas, No 7, 4485-661 Vairão, Portugal.
We performed a molecular and cytogenetic analysis on different Mantellinae species and revised the available chromosomal data on this group to provide an updated assessment of its karyological diversity and evolution. Using a fragment of the mitochondrial 16S rRNA, we performed a molecular taxonomic identification of the samples that were used for cytogenetic analyses. A comparative cytogenetic analysis, with Giemsa's staining, Ag-NOR staining and sequential C-banding + Giemsa + CMA + DAPI was performed on eight species: sp.
View Article and Find Full Text PDFVariations in heterochromatin content reveal important polymorphisms for studies of genetic improvement in garlic (Allium sativum L.).
Braz J Biol
June 2021
Universidade Federal do Piauí - UFPI, Centro de Ciências Agrárias - CCA, Programa de Pós-graduação em Agronomia - PPGA, Teresina, PI, Brasil.
Allium sativum L. is an herb of the Alliaceae family with a specific taste and aroma and medicinal and nutraceutical properties that are widely marketed in several countries. Brazil is one of the largest importers of garlic in the world, despite of its production is restricted and limited to internal consumption.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!