Objectives: Scutellaria baicalensis leaf (SLE), the above-ground part of the traditional Chinese medicine Scutellaria baicalensis Georgi, is rich in resources and contains a large number of flavonoids with anti-inflammatory, antioxidant and neuroprotective functions. The present study evaluated the ameliorative effects and related mechanisms of SLE on d-gal-induced ageing rats, providing a theoretical basis for the exploitation of SLE.
Methods: This experiment investigated the mechanism of SLE for anti-ageing by non-targeted metabonomics technology combined with targeted quantitative analysis and molecular biology technology.
Key Findings: Non-targeted metabonomics analysis showed that 39 different metabolites were screened out. Among them, 38 metabolites were regulated by SLE (0.4 g/kg), and 33 metabolites were regulated by SLE (0.8 g/kg). Through enrichment analysis, glutamine-glutamate metabolic pathway was identified as the key metabolic pathway. Subsequently, the results of targeted quantitative and biochemical analysis displayed that the contents of key metabolites and the activities of enzymes in glutamine-glutamate metabolic pathway and glutathione synthesis could be regulated by SLE. Furthermore, the results of Western blotting indicated that SLE significantly modulated the expression of Nrf2, GCLC, GCLM, HO-1, and NQO1 proteins.
Conclusion: To sum up, the anti-ageing mechanism of SLE was related to glutamine-glutamate metabolism pathway and Nrf2 signalling pathway.
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http://dx.doi.org/10.1093/jpp/rgad050 | DOI Listing |
Nutr Neurosci
December 2024
Exercise and Performance Nutrition Laboratory, College of Science, Technology and Health, Lindenwood University, St. Charles, MO, USA.
Unlabelled: Flavonoids, known for their neuroprotective properties, are abundant in and Yet, human studies on their combined effects are limited.
Objective: This study evaluated the cognitive effects of combined and supplementation in healthy adults.
Methods: In a randomized, double-blind, placebo-controlled trial, 26 males and 59 females ( = 85; 43 ± 8 yrs) consumed the test product (TP) containing 240 mg stem extract of and 51 mg heartwood extract of (UP326, Unigen, Tacoma, WA USA) or placebo (PLA) for four weeks.
Introduction: This study aimed to explore the impact and mechanism of Scutellariae radix (SR), dried root of Scutellaria baicalensis Georgi of Labiatae, on prenatal stress (PS) induced anxiety-like and depression-like behavior in the offspring in a mouse prenatal stress model.
Methods: The open field test (OFT), tail suspension test (TST), and forced swimming test (FST) were utilized to assess the behavior of the offspring. Histological changes were evaluated using HE staining and Nissl staining.
Talanta
December 2024
Center of Pharmaceutical Engineering and Technology, Harbin University of Commerce, Harbin, 150076, China. Electronic address:
Developing an accurate and ultrasensitive method for the simultaneous detection of baicalin and baicalein, two key flavonoid from Scutellaria baicalensis, is critical, particularly in the context of traditional Chinese medicine. This study focuses on an innovative electrochemical approach, utilizing a biochar (BC)/ZIF-67/MnCoO composite, which was fabricated via a combination of ultrasonic, calcination, and hydrothermal treatments. The resulting BC/ZIF-67/MnCoO electrode exhibits exceptional sensitivity, with linear response ranges of 0.
View Article and Find Full Text PDFHeliyon
December 2024
Department of Radiation Biology & Toxicology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal 576104, India.
Despite years of development in cancer therapy, achieving successful cancer treatment remains a major research topic. Primary means of cancer treatment include chemotherapy, radiotherapy, and surgery. However, these modalities are associated with limitations and adverse effects on normal tissues.
View Article and Find Full Text PDFFront Pharmacol
December 2024
Department of Clinical Pharmacy, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Neurodegenerative diseases (NDDs) represent a category of serious illnesses characterized by the progressive deterioration of neuronal structure and function. The exploration of natural compounds as potential therapeutic agents has gained increasing attention in recent years owing to their wide range of pharmacological activities and minimal side effects. Baicalin (BAI) and baicalein (BE), polyphenolic flavonoids, derived from the root of , evidently show potential in treating NDDs.
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