A Gram-stain-negative, aerobic, reddish-coloured, rod-shaped and non-motile strain PAMC 29467, was isolated from freshwater of the pond in Cambridge Bay, Canada. Strain PAMC 29467 was closely related to (98.1 % 16S rRNA gene similarity). Genomic relatedness analyses showed that strain PAMC 29467 is distinguishable from based on average nucleotide identity (91.3 %) and digital DNA-DNA hybridization values (39.3 %). The major fatty acids (>10 %) of strain PAMC 29467 were summed feature 3 (C 7 and/or C 6), C iso, C 5 and summed feature 4 (C iso l and/or anteiso B). The major respiratory quinone was menaquinone-7. The genomic DNA G+C content was 61.5 mol%. Strain PAMC 29467 was separated from the type species in the genus by its distinct phylogenetic position and some physiological characteristics. As a result, a novel species is proposed, with the name sp. nov. (type strain, PAMC 29467=KCTC 92787=JCM 35843).
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http://dx.doi.org/10.1099/ijsem.0.005913 | DOI Listing |
Antonie Van Leeuwenhoek
November 2024
School of Basic Medical Sciences, Zunyi Medical University, Zunyi, 563006, People's Republic of China.
Antonie Van Leeuwenhoek
September 2024
Division of Life Sciences, Korea Polar Research Institute, 26 Songdomirae-ro, Yeonsu-gu, Incheon, 21990, Republic of Korea.
J Agric Food Chem
August 2024
Anhui Provincial Key Laboratory of Hazardous Factors and Risk Control of Agri-food Quality Safety, College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China.
Propanil residues can contaminate habitats where microbial degradation is predominant. In this study, an efficient propanil-degrading strain C-1 was isolated from paddy and identified as sp. It can completely degrade 10 μg/L-150 mg/L propanil within 0.
View Article and Find Full Text PDFHeliyon
June 2024
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Two novel endophytic bacterial strains, designated KSS8 and KSS12, were isolated from the stems and roots of sugarcane, respectively, collected in Nakhon Ratchasima, Thailand. They were Gram-stain-negative, aerobic, and rod-shaped. The strain KSS8 was a motile bacterium with a subpolar flagellum, while the strain KSS12 was non-motile.
View Article and Find Full Text PDFProtein Expr Purif
December 2023
Research Unit of Cryogenic Novel Material, Korea Polar Research Institute, Incheon, 21990, Republic of Korea; Department of Polar Sciences, University of Science and Technology, Incheon, 21990, Republic of Korea. Electronic address:
Insolubility and low expression are typical bottlenecks in the production of proteins for studying their function and structure using X-ray crystallography or nuclear magnetic resonance spectroscopy. Cold-active enzymes from polar microorganisms have unique structural features that render them unstable and thermolabile, and are responsible for decreased protein yield in heterologous expression systems. To address these challenges, we developed a heterologous protein expression system using a psychrophilic organism, Psychrobacter sp.
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