Antibiotics Resistance and Adhesive Properties of Clinical Isolated from Wound Infections.

() is a ubiquitous pathogen responsible for several severe infections. This study aimed to investigate the adhesive properties and antibiotic resistance among clinical isolated from Hail Hospital Province, Kingdom of Saudi Arabia (KSA), using molecular approaches. This study was conducted according to the ethical committee at Hail's guidelines on twenty-four isolates. A polymerase chain reaction (PCR) was performed to identify genes encoding the β-lactamase resistance (Z), methicillin resistance (A), fluoroquinolone resistance (A), nitric oxide reductase (B), fibronectin (A and B), clumping factor (A) and intracellular adhesion factors (A and D). This qualitative study tested adhesion based on exopolysaccharide production on Congo red agar (CRA) medium and biofilm formation on polystyrene by strains. Among 24 isolates, the and blaz were the most prevalent (70.8%), followed by B (54.1%), A (50.0%), A (41.6%), A and B (37.5%) and A (33.3%). The presence of A/D genes was demonstrated in almost all tested strains in comparison to the reference strain, ATCC 43300. The phenotypic study of adhesion showed that all tested strains had moderate biofilm-forming capacity on polystyrene and represented different morphotypes on a CRA medium. Five strains among the twenty-four harbored the four genes of resistance to antibiotics (A, A, B and z). Considering the genes of adhesion (, A, A and B), these genes were present in 25% of the tested isolates. Regarding the adhesive properties, the clinical isolates of formed biofilm on polystyrene, and only one strain (S17) produced exopolysaccharides on Congo red agar. All these results contribute to an understanding that the pathogenesis of clinical isolates is due to their antibiotic resistance and adhesion to medical material.