Background: is associated with milk, dairy product, and dairy farm contamination. The aim of this study was to characterize strains of in the small-scale artisanal cheese production chain in southwestern Mexico.
Methods: 130 samples were collected. isolation was performed on Mannitol Egg Yolk Polymyxin (MYP) agar. Genotyping, enterotoxigenic profile, and determination of genes involved in the formation of biofilm were performed by PCR. An antimicrobial susceptibility test was made by broth microdilution assay. The phylogenetic analysis was performed by amplification and sequencing of 16s rRNA.
Results: was isolated and molecularly identified in 16 samples and () was the most frequently isolated and identified species (81.25%). Of all the isolated strains, 93.75% presented at least one gene for some diarrheagenic toxins, 87.5% formed biofilms, and 18.75% were amylolytic. All strains were resistant to beta-lactams and folate inhibitors. A close phylogenetic relationship between isolates was found between the cheese isolates and the air isolates.
Conclusions: Strains of were found in small-scale artisanal cheeses on a farm in southwestern Mexico.
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| http://dx.doi.org/10.3390/microorganisms11051290 | DOI Listing |
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