AI Article Synopsis

  • * A custom electrochemical reactor was used to modify RBP, revealing significant changes in protein structure and morphology, such as decreased α-helix and β-sheet content, and increased free sulfhydryl groups.
  • * The modifications led to improved emulsification properties, including a decrease in particle size, enhanced emulsification capacity (65.82 m/g), and stability (36.34 min), indicating successful alkylation of RBP.

Article Abstract

To replace the application of reducing agents for protein alkylation, electrochemical reduction techniques have been considered. In this study, a custom-made electrochemical reactor was used to alkylate rice bran protein (RBP). The structure, morphology, and emulsification properties of RBP were investigated under different voltages. When treated at 35 V, the content of the α-helix and β-sheet of RBP decreased at first and then increased, whereas the content of the β-turn and random coil increased steadily. The CH group of RBP was exposed and S-S decreased. The endogenous fluorescence spectral curve exhibited a redshift. The free sulfhydryl (-SH) content increased. The average particle size of the modified RBP decreased by 69.35 %, and its zeta potential decreased to -21.8 mV. Atomic force microscopy (AFM) revealed that the treated protein particles dispersed more evenly and their roughness (Rq) decreased. The contact angle, water holding capacity (WHC), fat holding capacity (FHC), and solubility were enhanced. The emulsification capacity increased to 65.82 m/g and emulsification stability increased to 36.34 min. These results demonstrated that the RBP was alkylated by the electrochemical reactor and the modified RBP showed improved emulsification properties compared to the untreated RBP.

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Source
http://dx.doi.org/10.1016/j.foodres.2023.112561DOI Listing

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