Pinpointing for frogeye leaf spot resistance and tracing its origin in soybean breeding.

Unlabelled: Frogeye leaf spot is a yield-reducing disease of soybean caused by the pathogen . has provided durable resistance to all known races of since its discovery in the cultivar Davis during the 1980s. Using a recombinant inbred line population derived from a cross between Davis and the susceptible cultivar Forrest, was fine-mapped to a 1.15 Mb interval on chromosome 16. This single locus was confirmed by tracing in resistant and susceptible progeny derived from Davis, as well as three near-isogenic lines. Haplotype analysis in the ancestors of Davis indicated that Davis has the same haplotype at the locus as susceptible cultivars in its paternal lineage. On the basis of these results, it is hypothesized that the resistance allele in Davis resulted from a mutation of a susceptibility allele. Tightly linked SNP markers at the locus identified in this research can be used for effective marker-assisted selection.

Supplementary Information: The online version contains supplementary material available at 10.1007/s11032-023-01397-x.