Analysis of -Associated ceRNA Network Reveals Oil Metabolism in Soybean.

Soybean [ (Linn.) Merr.] is an important oil crop. Long noncoding RNAs (lncRNAs) play a variety of functions in plants. However, their function in the soybean oil synthesis pathway is yet to be uncovered. Here, the gene related to soybean oil synthesis was screened, and the full-length cDNA sequence of the lncRNA was obtained using rapid amplification of cDNA ends. Overexpression of increased the content of crude protein in seeds, decreased the content of oleic acid, and affected the content of alanine and arginine in free amino acids. RNA interference of the gene decreased the crude protein content in seeds. Quantitative real-time polymerase chain reaction analysis revealed that influenced the expression of associated with phosphatidylinositol metabolism by acting as a decoy for , thereby affecting the content of soybean oil. Our results provide insights into how lncRNA-mediated competing endogenous RNA regulatory networks are involved in soybean oil synthesis.