Keratinocytes are the predominant cell type in the skin epidermis, and they not only protect the skin from the influence of external physical factors but also function as an immune barrier against microbial invasion. However, little is known regarding the immune defence mechanisms of keratinocytes against mycobacteria. Here, we performed single-cell RNA sequencing (scRNA-seq) on skin biopsy samples from patients with Mycobacterium marinum infection and bulk RNA sequencing (bRNA-seq) on M. marinum-infected keratinocytes in vitro. The combined analysis of scRNA-seq and bRNA-seq data revealed that several genes were upregulated in M. marinum-infected keratinocytes. Further in vitro validation of these genes by quantitative polymerase chain reaction and western blotting assay confirmed the induction of IL-32 in the immune response of keratinocytes to M. marinum infection. Immunohistochemistry also showed the high expression of IL-32 in patients' lesions. These findings suggest that IL-32 induction is a possible mechanism through which keratinocytes defend against M. marinum infection; this could provide new targets for the immunotherapy of chronic cutaneous mycobacterial infections.
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http://dx.doi.org/10.1111/exd.14848 | DOI Listing |
J Infect Dev Ctries
November 2024
Department of Clinical Laboratory, Shenzhen Qianhai Shekou Free Trade Zone Hospital, Shenzhen, China.
Introduction: Mycobacterium marinum is a well-known pathogenic non-tuberculous mycobacterium for skin and soft tissue infections. Infection, often presenting as superficial lesions, is seen after exposure of skin abrasions to contaminated water or infected fish and is known as "swimming pool" or "fish tank" granuloma. This study reported a case of M.
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November 2024
Dermatology, University of Occupational and Environmental Health, Kitakyushu, JPN.
Acta Derm Venereol
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Department of Dermatology, Skåne University Hospital, Malmö, Sweden; Department of Clinical Sciences, Faculty of Medicine, Lund University, Malmö, Sweden.
Am J Case Rep
November 2024
Department of Infectious Diseases, St. Luke's International Hospital, Tokyo, Japan.
Sci Rep
November 2024
College of Medicine, Qatar University (QU) Health, Qatar University, Doha, Qatar.
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