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Evaluation of tomato brown rugose fruit virus as a microbial source tracking marker for human sewage in Thailand.
Sci Total Environ
January 2025
Research Laboratory of Biotechnology, Chulabhorn Research Institute, Bangkok 10210, Thailand; Center of Excellence on Environmental Health and Toxicology (EHT), OPS, MHESI, Bangkok, Thailand. Electronic address:
Tomato brown rugose fruit virus (ToBRFV) has emerged as a major plant pathogen with the potential to spread through contaminated wastewater, posing risks to agriculture and public health. This study evaluated ToBRFV as a human-specific microbial source tracking (MST) marker in Thailand, comparing its performance to crAssphage. Using qPCR assays, ToBRFV was detected in 62.
View Article and Find Full Text PDFBacterial Wastewater-Based Epidemiology Using Surface-Enhanced Raman Spectroscopy and Machine Learning.
Nano Lett
January 2025
Department of Materials Science and Engineering, Stanford University, Stanford, California 94305, United States.
Although wastewater-based epidemiology has been used extensively for the surveillance of viral diseases, it has not been used to a similar extent for bacterial diseases. This is in part owing to difficulties in distinguishing pathogenic from nonpathogenic bacteria using PCR methods. Here, we show that surface-enhanced Raman spectroscopy (SERS) can be a scalable, label-free method for the detection of bacteria in wastewater.
View Article and Find Full Text PDFToward the elimination of NTDs: application of cost-effective and sensitive molecular environmental surveillance tools-a pilot study.
Front Parasitol
March 2024
Biomedical and Public Health Research Unit, Council for Scientific and Industrial Research -Water Research Institute, Accra, Ghana.
Neglected tropical diseases (NTDs) affect over a billion people worldwide. The 2021-2030 NTD road map calls for innovative and highly efficient interventions to eliminate or significantly reduce the burden of NTDs. These include sensitive and cost-effective diagnostic techniques for disease surveillance.
View Article and Find Full Text PDFSpatiotemporal Variability of the Pepper Mild Mottle Virus Biomarker in Wastewater.
ACS ES T Water
January 2025
Department of Statistics & Data Science, Dietrich College of Humanities and Social Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, United States.
Since the start of the coronavirus-19 pandemic, the use of wastewater-based epidemiology (WBE) for disease surveillance has increased throughout the world. Because wastewater measurements are affected by external factors, processing WBE data typically includes a normalization step in order to adjust wastewater measurements (e.g.
View Article and Find Full Text PDFActivated sludge prokaryote and eukaryote characterization in a pulp mill facility using amplicon sequencing.
Heliyon
July 2024
Bioinformatics Unit, Institut Pasteur de Montevideo, Montevideo, Uruguay.
The pulp and paper industry, a major global sector, supports economies and jobs while contributing to various products. While providing valuable products, and despite Best Available Techniques (BAT) being used, managing wastewater effectively remains a key area for developing technologies and alternatives for environmental protection. Activated sludge (AS) systems are commonly used for effluent treatment, where microorganisms composition influences reactor efficiency.
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