AI Article Synopsis
- Vascular calcification is a systemic issue where calcium builds up in blood vessel walls, requiring a sophisticated in vitro model for study.
- The protocol outlines creating a dynamic co-culture system using endothelial and smooth muscle cells to simulate real vascular tissue in a lab setting.
- Key steps include cell culture, using a double-flow bioreactor to mimic blood flow, inducing calcification, and assessing cell health and calcium levels.
Article Abstract
Vascular calcification is a systemic disease characterized by calcium salt deposition within vascular walls. Here, we present a protocol for establishing an advanced dynamic in vitro co-culture system using endothelial and smooth muscle cells to replicate vascular tissue complexity. We describe steps for cell culture and seeding in a double-flow bioreactor that recreates the action of blood in humans. We then detail the induction of calcification, setting up of the bioreactor, followed by cell viability assessment and calcium quantification.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10323150 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2023.102328 | DOI Listing |
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