AI Article Synopsis
- Surface plasmon resonance (SPR) sensing is introduced as a method for real-time detection of biomolecular interactions on gold surfaces, using a novel nano-diamond (ND) approach.
- In this study, anti-bovine serum albumin (anti-BSA) is bound to NDs, which are then coupled to a gold nano-slit array, allowing the assessment of biomolecule concentration through shifts in the extraordinary transmission (EOT) spectrum.
- The use of 35 nm NDs significantly enhances signal responses—approximately 10-fold compared to anti-BSA alone—while allowing for lower analyte concentrations, making this technique efficient for biochip applications.
Article Abstract
Surface plasmon resonance (SPR) sensing is a real-time detection technique for measuring biomolecular interactions on gold surfaces. This study presents a novel approach using nano-diamonds (NDs) on a gold nano-slit array to obtain an extraordinary transmission (EOT) spectrum for SPR biosensing. We used anti-bovine serum albumin (anti-BSA) to bind NDs for chemical attachment to a gold nano-slit array. The covalently bound NDs shifted the EOT response depending on their concentration. The number of ND-labeled molecules attached to the gold nano-slit array was quantified from the change in the EOT spectrum. The concentration of anti-BSA in the 35 nm ND solution sample was much lower than that in the anti-BSA-only sample (approximately 1/100). With the help of 35 nm NDs, we were able to use a lower concentration of analyte in this system and obtained better signal responses. The responses of anti-BSA-linked NDs had approximately a 10-fold signal enhancement compared to anti-BSA alone. This approach has the advantage of a simple setup and microscale detection area, which makes it suitable for applications in biochip technology.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10256044 | PMC |
| http://dx.doi.org/10.3390/s23115216 | DOI Listing |
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Article Synopsis
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