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Babesiosis in sickle cell disease (SCD) is marked by severe anemia but the underlying red blood cell (RBC) rheological parameters remain largely undefined. Here, we describe altered RBC deformability from both primary (host RBC sickle hemoglobin mediated) and secondary changes (Babesia parasite infection mediated) to the RBC membrane using wild type AA, sickle trait AS and sickle SS RBCs. Our ektacytometry (LORRCA) analysis demonstrates that the changes in the host RBC bio-mechanical properties, pre- and post- Babesia infection, reside on a spectrum of severity, with wild type infected AA cells, despite showing a significant reduction of deformability under both shear and osmolarity gradients, exhibiting only a mild phenotype; compared to infected AS RBCs which show median changes in deformability and infected SS RBCs which exhibit the most dramatic impact of infection on cellular rheology, including an increase in Point of Sickling values.

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Probing the interaction of mannose-binding lectin with healthy and sickle cell anemia red blood cells and its role in cellular biomechanics.

Int J Biol Macromol

January 2025

Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, Pernambuco 50670-901, Brazil. Electronic address:

Mannose-binding lectin (MBL) is an important glycoprotein of the human innate immune system. Furthermore, individuals with sickle cell anemia (SCA) and MBL deficiency seem more susceptible to vaso-occlusive crises, suggesting an MBL role on HbSS red blood cells (RBCs). This study investigated the interaction of MBL with HbA (healthy) and HbSS RBCs using optical tweezers (OT) and atomic force microscopy (AFM).

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The present study aimed to evaluate the physiological responses to transport stress in juvenile tambaqui () fed a diet supplemented with hydroxy-selenomethionine (OH-SeMet; Selisseo, Adisseo) and determine through stress biomarkers whether selenium supplementation could reduce the impact of transport stress on tambaqui resilience. Juvenile fish (15.71 ± 1.

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Cellular and chromosomal interaction of bio-synthesized copper oxide nanoparticles - Induced nano-cytotoxicity and genotoxicity.

Toxicol In Vitro

December 2024

Nanodrug Co-Delivery Systems Laboratory, Department of Nanoscience and Technology, Bharathiar University, Coimbatore 641046, Tamil Nadu, India. Electronic address:

The widespread use of nanoparticles raises substantial environmental, health, and safety issues. The specific mechanisms by which they impact plants and animals, as well as the entire scope of their possible impact, are still unknown. The current work investigates the impact of varying CuO NPs concentrations on phytotoxicity, cytotoxicity, genotoxicity, and antioxidant activity.

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Background: Thalassemia, a genetic blood disorder, poses significant global health challenges, emphasizing the importance of accurate screening methods. Traditional diagnostic tools, such as osmotic fragility and dichlorophenolindophenol tests, along with blood indices, such as mean corpuscular volume and mean corpuscular hemoglobin, have limitations. Digital microscopy of peripheral blood smears is a promising alternative for objective quantification and standardization.

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