AI Article Synopsis
- Global climate change and international trade are increasing the risk of introducing new plant viruses across borders, as seen in the case of Ixora coccinea showing virus-like symptoms.
- The Oxford Nanopore Technologies MinION platform was used to identify jasmine virus H (JaVH), revealing a complete genome sequence that closely resembles a variant found in Jasminum sambac from China.
- This study marks the first reported natural infection of Ixora coccinea by JaVH and highlights the effectiveness of rapid nanopore sequencing for accurate plant virus detection and surveillance.
Article Abstract
The global climate change and international trade have facilitated the movement of plants across borders, increasing the risk of introducing novel plant viruses in new territories. Ixora coccinea exhibited virus-like foliar symptoms, including mosaic and mild mottle. An Oxford Nanopore Technologies-based compact and portable MinION platform was used to identify the causal viral pathogen. The complete genome sequence of jasmine virus H (JaVH; 3867 nt, JaVH-CNU) was determined and found to share 88.4-90.3% nucleotide identity with that of Jasminum sambac JaVH isolate in China. Phylogenetic analysis based on the complete amino acid sequences of RNA-dependent RNA polymerase and coat protein revealed that JaVH-CNU was grouped separately with other JaVH isolates. This is the first report of a natural JaVH infection of >i<I. coccinea. The application of rapid nanopore sequencing for plant virus identification was demonstrated and is expected to provide accurate and rapid diagnosis for virus surveillance.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10265114 | PMC |
| http://dx.doi.org/10.5423/PPJ.NT.03.2023.0037 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Complete genome sequence analysis of a novel nepovirus isolated from Jasminum polyanthum in Yunnan, China.
Arch Virol
December 2024
Key Laboratory for Agricultural Bioaffiliationersity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, NO. 95, Jinhei Road, Panlong District, Kunming, Yunnan, 650201, China.
Here, we report the complete genome sequence of Jasminum polyanthum nepovirus 1 (JPV1), a novel virus associated with foliar ringspot symptoms in Jasminum polyanthum (Oleaceae). The genome of JPV1 consists of RNA1 and RNA2, both of which are monocistronic and are 7081 nt and 4212 nt in length, respectively. RNA1 encodes six functional proteins, including the X1 protein, X2 protein, NTP-binding protein (NTB), VPg protein, protease cofactor (Pro), and RNA-dependent RNA polymerase (RdRp), while RNA2 encodes three functional proteins: the 2A protein, movement protein (MP), and capsid protein (CP).
View Article and Find Full Text PDFGolden Therapeutic Approach to Combat Viral Diseases Using Gold Nanomaterials.
Assay Drug Dev Technol
December 2024
Amity Institute of Pharmacy, Amity University Uttar Pradesh, Noida, India.
Advanced Lung Disease Detection: CBAM-Augmented, Lightweight EfficientNetB2 with Visual Insights.
Curr Med Imaging
December 2024
School of Computer Science and Engineering, Vellore Institute of Technology, Chennai, India.
Introduction: This paper presents a multichannel deep-learning method for detecting lung diseases using chest X-ray images. Using EfficientNetB0 through EfficientNetB7 pretrained models, the methodology offers improved performance in classifying COVID-19, viral pneumonia, and normal chest Xrays.
Methods: The EfficientNetB2 model was customized by incorporating Squeeze-and-Excitation (SE) blocks and the Convolutional Block Attention Module (CBAM) to improve the model's attention mechanisms.
Trends in Urinary Tract Infection Management in Women.
Obstet Gynecol
January 2025
Division of Urogynecology and Reconstructive Pelvic Surgery, Department of Obstetrics & Gynecology, Kaiser Permanente Southern California, and the Division of Urogynecology and Reconstructive Pelvic Surgery, Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Diego, San Diego, and the Department of Research and Evaluation, Kaiser Permanente, Pasadena, California.
Article Synopsis
- Urinary tract infections (UTIs) led to a significant rise in health care visits in the U.S., especially during the COVID-19 pandemic, which boosted the use of virtual care for these issues.* -
- A study analyzing over 1.2 million UTI encounters from 2015 to 2022 found a 325.9% increase in UTI visits, predominantly through virtual consultations, particularly among adult females.* -
- The research noted that while antibiotic prescriptions rose, they outpaced the rate of confirmed positive urine cultures, indicating a trend towards more empirical antibiotic use and emphasizing the need for better guidelines to balance telemedicine with proper antibiotic stewardship.*
analysis of quercetin-like compounds from mistletoe e as a potential antiviral agent for Newcastle disease.
F1000Res
July 2024
Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam 13200 Kepala Batas, Penang, Penang, 13200, Malaysia.
Background: Recent evidence suggests that some flavonoid compounds obtained from crude methanol extract of mistletoe leaves ( ), also known as Benalu Duku (BD), have antimicrobial effects. Thus, the plant has the potential to eliminate viruses that may cause outbreaks in chicken farms. This study aimed to prove the ability of flavonoid compounds, namely quercetin-like compounds (QLCs), to eliminate field viruses, specifically the Newcastle disease virus (NDV).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!