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Nuclear segmentation facilitates neutrophil migration. | LitMetric

Nuclear segmentation facilitates neutrophil migration.

J Cell Sci

Department of Respiratory Medicine, Center of Translational Immunology, University Medical Center Utrecht, 3584 EA, Utrecht, The Netherlands.

Published: June 2023

AI Article Synopsis

  • * Researchers used custom microfluidic devices to observe neutrophil movement in narrow channels after administering low-dose endotoxin to recruit diverse neutrophils with different nuclear shapes.
  • * The study found that neutrophils with one or two lobes migrated slower through narrow spaces than those with more than two lobes, indicating that nuclear segmentation enhances their migration speed.

Article Abstract

Neutrophils are among the fastest-moving immune cells. Their speed is critical to their function as 'first responder' cells at sites of damage or infection, and it has been postulated that the unique segmented nucleus of neutrophils functions to assist their rapid migration. Here, we tested this hypothesis by imaging primary human neutrophils traversing narrow channels in custom-designed microfluidic devices. Individuals were given an intravenous low dose of endotoxin to elicit recruitment of neutrophils into the blood with a high diversity of nuclear phenotypes, ranging from hypo- to hyper-segmented. Both by cell sorting of neutrophils from the blood using markers that correlate with lobularity and by directly quantifying the migration of neutrophils with distinct lobe numbers, we found that neutrophils with one or two nuclear lobes were significantly slower to traverse narrower channels, compared to neutrophils with more than two nuclear lobes. Thus, our data show that nuclear segmentation in primary human neutrophils provides a speed advantage during migration through confined spaces.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10309577PMC
http://dx.doi.org/10.1242/jcs.260768DOI Listing

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