AI Article Synopsis

  • - Several antibody therapeutics exist for SARS-CoV-2, but their effectiveness has decreased against emerging variants, prompting researchers to explore new antibodies obtained from recovered COVID-19 patients' B cells.
  • - From 172 antibodies created using the Wuhan strain and Gamma variant, six were effective against earlier strains, while five showed some ability to combat Omicron sub-strains, indicating a potential for broader neutralization.
  • - Testing one promising antibody in hamsters showed a significant reduction in lung viral levels, demonstrating its potential as an antiviral treatment and underscoring the need for effective initial screening in developing such therapies.

Article Abstract

Several antibody therapeutics have been developed against SARS-CoV-2; however, they have attenuated neutralizing ability against variants. In this study, we generated multiple broadly neutralizing antibodies from B cells of convalescents, by using two types of receptor-binding domains, Wuhan strain and the Gamma variant as bait. From 172 antibodies generated, six antibodies neutralized all strains prior to the Omicron variant, and the five antibodies were able to neutralize some of the Omicron sub-strains. Structural analysis showed that these antibodies have a variety of characteristic binding modes, such as ACE2 mimicry. We subjected a representative antibody to the hamster infection model after introduction of the N297A modification, and observed a dose-dependent reduction of the lung viral titer, even at a dose of 2 mg/kg. These results demonstrated that our antibodies have certain antiviral activity as therapeutics, and highlighted the importance of initial cell-screening strategy for the efficient development of therapeutic antibodies.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10208659PMC
http://dx.doi.org/10.1016/j.isci.2023.106955DOI Listing

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