AI Article Synopsis
- Anaerobic microbial degradation of hydrocarbons begins when enzymes known as X-succinate synthases (XSSs) add hydrocarbons to fumarate, using a special cofactor activated by XSS-AE.
- Genome mining helped identify a soluble XSS-AE (IbsAE) that can activate both IBSS and the more familiar benzylsuccinate synthase (BSS), facilitating biochemical studies of XSSs.
- Research indicates that the beta subunit of BSS speeds up the hydrocarbon addition process, presenting opportunities to further explore and engineer XSSs as valuable biocatalysts.
Article Abstract
Anaerobic microbial degradation of hydrocarbons is often initiated through addition of the hydrocarbon to fumarate by enzymes known as X-succinate synthases (XSSs). XSSs use a glycyl radical cofactor, which is installed by an activating enzyme (XSS-AE), to catalyze this carbon-carbon coupling reaction. The activation step, although crucial for catalysis, has not previously been possible because of insolubility of XSS-AEs. Here, we take a genome mining approach to find an XSS-AE, a 4-isopropylbenzylsuccinate synthase (IBSS)-AE (IbsAE) that can be solubly expressed in . This soluble XSS-AE can activate both IBSS and the well-studied benzylsuccinate synthase (BSS) , allowing us to explore XSSs biochemically. To start, we examine the role of BSS subunits and find that the beta subunit accelerates the rate of hydrocarbon addition. Looking forward, the methodology and insight gathered here can be used more broadly to understand and engineer XSSs as synthetically useful biocatalysts.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10239695 | PMC |
| http://dx.doi.org/10.1016/j.isci.2023.106902 | DOI Listing |
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Article Synopsis
- Anaerobic microbial degradation of hydrocarbons begins when enzymes known as X-succinate synthases (XSSs) add hydrocarbons to fumarate, using a special cofactor activated by XSS-AE.
- Genome mining helped identify a soluble XSS-AE (IbsAE) that can activate both IBSS and the more familiar benzylsuccinate synthase (BSS), facilitating biochemical studies of XSSs.
- Research indicates that the beta subunit of BSS speeds up the hydrocarbon addition process, presenting opportunities to further explore and engineer XSSs as valuable biocatalysts.
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