Identification of cloned cells is necessary for experimentation with them. This paper details a method for the identification of cultured human malignant prostatic epithelial cells derived from metastatic deposits of prostate cancer by localization of a specific rabbit antiserum to human prostatic acid phosphatase in the cells.
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JACC Case Rep
January 2025
Department of Cardiology and Nephrology, Mie University Graduate School of Medicine, Tsu, Japan.
can cause culture-negative endocarditis, and almost all antibiotics included in empirical therapy regimens for endocarditis are ineffective against this pathogen. We report a case of culture-negative endocarditis in which identification of the pathogen avoided repeated valve destruction and surgery.
View Article and Find Full Text PDFACS Sens
January 2025
Department of Chemistry, Chung-Ang University, Seoul 06974, South Korea.
Bacterial infections, such as sepsis, require prompt and precise identification of the causative bacteria for appropriate antibiotics treatment. Traditional methods such as culturing take 2-5 days, while newer techniques such as reverse transcription-polymerase chain reaction and mass spectrometry are hindered by blood impurities. Consequently, this study developed a surface-enhanced Raman scattering (SERS)-based acoustofluidic technique for rapid bacterial detection without culturing or lysing.
View Article and Find Full Text PDFRev Argent Microbiol
January 2025
Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:
Despite conducting studies to investigate food contamination in hospitals in different parts of Iran in recent years, there have been no reliable studies to identify Salmonella enteritidis, Salmonella typhimurium, Bacillus cereus, Bacillus subtilis, and Clostridium perfringens in hospital food in Mashhad. Therefore, this study was conducted with the aim of investigating some major foodborne pathogens in hospital food. In this study, 360 food samples were randomly selected from 12 different menus from 13 hospitals affiliated with Mashhad University of Medical Sciences, Mashhad, Iran.
View Article and Find Full Text PDFPLoS One
January 2025
Health Promotion Sciences Department, Mel and Enid Zuckerman College of Public Health, University of Arizona, Tucson, Arizona, United States of America.
The complex healthcare system in the United States (US) poses significant challenges for people, particularly minorities such as refugees. Refugees often encounter additional layers of challenges to healthcare navigation due to unfamiliarity with the system, limited health literacy, and language barriers. Despite their challenges, it is difficult to identify the gaps as few tools exist to measure navigation competency among this population and many conventional tools assume English proficiency, making them inadequate for refugees and other immigrants.
View Article and Find Full Text PDFPlant Dis
January 2025
Huainan Normal University, School of Bioengineering, Dongshan West Road, Huainan City, Huainan, China, 232038;
Manglietia decidua is an extremely endangered species, known for its limited population and a narrow distribution range restricted to China (Yu 1994). In October 2021, a leaf disease affecting the foliage of 3-year-old M. decidua was observed at the nursery garden of the Yichun Forestry Institute of Jiangxi Province (27°55'52.
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