A simple procedure is described for removing the jelly and vitelline membrane of Xenopus laevis embryos. The method is based on the observation that incubation of the embryos in the mixed solution of trypsin and sodium thioglycolate at pH 8.0 causes effective dissolution of these structures. This solution is equally effective in this respect on the embryos at different developmental stages. Normal development is obtained from all of the denuded neurulae and from many of the denuded earlier embryos. Some chemical properties of the jelly and the vitelline membrane of Xenopus laevis are discussed based upon these observations.
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| http://dx.doi.org/10.1111/j.1440-169X.1972.00037.x | DOI Listing |
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