A method for joining larvae in the starfish, Asterina pectinifera, is presented. Any number of embryos are stably united by simple contact of the cell clusters (descendents of individual denuded eggs) before reaching the early blastula stage (ca. 6 1/2 hr after insemination at 21°C). Embryos do not combine with each other after closing into hollow blastulae. This is considered to indicate the transformation of the cell cluster from a mere collection of cells to an individual, multicellular system. Biological meanings of the fertilization membrane are discussed.
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| http://dx.doi.org/10.1111/j.1440-169X.1977.00233.x | DOI Listing |
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