AI Article Synopsis

  • AQP8 is a protein that is found in higher amounts in brain tumors called gliomas compared to normal brain tissue, and it seems to help these tumors grow and spread.
  • Scientists used special techniques to change the amount of AQP8 in certain glioma cells and studied how it affected the cells' growth and movement.
  • They discovered that having more AQP8 made glioma cells grow and spread more, while reducing AQP8 had the opposite effect, suggesting that AQP8 could be an important target for treating gliomas.

Article Abstract

Background: The aquaporin (AQP) family of proteins has been implicated in the proliferation and growth of gliomas. Expression of AQP8 is higher in human glioma tissues than in normal brain tissues and is positively correlated with the pathological grade of glioma, suggesting that this protein is also involved in the proliferation and growth of glioma. However, the mechanism by which AQP8 promotes the proliferation and growth of glioma remains unclear. This study aimed to investigate the mechanism and role of abnormal AQP8 expression in glioma development.

Methods: The dCas9-SAM and CRISPR/Cas9 techniques were used to construct viruses with overexpressed and knocked down AQP8, respectively, and infect A172 and U251 cell lines. The effects of AQP8 on the proliferation and growth of glioma and its mechanism via the intracellular reactive oxygen species (ROS) level were observed using cell clone, transwell, flow cytometry, Hoechst, western blotting, immunofluorescence, and real-time quantitative polymerase chain reaction assays. A nude mouse tumor model was also established.

Results: Overexpression of AQP8 resulted in an increased number of cell clones and cell proliferation, enhanced cell invasion and migration, decreased apoptosis and phosphatase and tensin homolog (PTEN) expression, and increased phosphorylated serine/threonine protein kinase (p-AKT) expression and ROS level, whereas the AQP8 knockdown groups showed opposite results. In the animal experiments, the AQP8 overexpression group had higher tumor volume and weight, whereas the AQP8 knockdown group had lower tumor volume and weight compared with those parameters measured in the control group.

Conclusions: Our results preliminary suggest that AQP8 overexpression alters the ROS/PTEN/AKT signaling pathway, promoting the proliferation, migration, and invasion of gliomas. Therefore, AQP8 may be a potential therapeutic target in gliomas.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10242804PMC
http://dx.doi.org/10.1186/s12885-023-11025-8DOI Listing

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