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Generation of LexA enhancer-trap lines in Drosophila by an international scholastic network. | LitMetric

AI Article Synopsis

  • The study focuses on the use of the LexA-LexAop system in Drosophila to explore gene and tissue function through newly developed LexA enhancer traps.
  • Researchers created and analyzed 301 novel Stan-X LexA enhancer trap insertions in various chromosomal locations, including some not previously linked to enhancer traps.
  • The initiative involved collaboration among high school and university students, emphasizing hands-on experimental science and improving access to genetics research for underrepresented student groups.

Article Abstract

Conditional gene regulation in Drosophila through binary expression systems like the LexA-LexAop system provides a superb tool for investigating gene and tissue function. To increase the availability of defined LexA enhancer trap insertions, we present molecular, genetic, and tissue expression studies of 301 novel Stan-X LexA enhancer traps derived from mobilization of the index SX4 line. This includes insertions into distinct loci on the X, II, and III chromosomes that were not previously associated with enhancer traps or targeted LexA constructs, an insertion into ptc, and seventeen insertions into natural transposons. A subset of enhancer traps was expressed in CNS neurons known to produce and secrete insulin, an essential regulator of growth, development, and metabolism. Fly lines described here were generated and characterized through studies by students and teachers in an international network of genetics classes at public, independent high schools, and universities serving a diversity of students, including those underrepresented in science. Thus, a unique partnership between secondary schools and university-based programs has produced and characterized novel resources in Drosophila, establishing instructional paradigms devoted to unscripted experimental science.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10468311PMC
http://dx.doi.org/10.1093/g3journal/jkad124DOI Listing

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