Purpose: This research aims to identify the miRNAs that could target the genes overexpressed in prostate cancer so that miRNA-based therapeutics could be developed.
Methods: A 7mer-m8 model of microRNA targeting was utilized in order to analyse the relationship between microRNAs and overexpressed genes. The efficiency of miRNA binding was investigated using various parameters namely free energy (AMFE), GC and GC3 content, translation efficiency, cosine similarity metric, mRNA stability, free energy of RNA duplex, and base compositional difference. BLAST2GO software was used to elucidate the functional roles of the genes overexpressed in prostate cancer.
Results: The current research reveals that the coding sequences of the genes were found targeted with multiple miRNAs. For instance, the HPN gene was targeted by the microRNA miR-4279 at two distinct sites i.e. 263-278 and 746-761 in the coding sequence. In the present study, it was observed that the target region of the genes exhibited a comparatively high GC and GC3 contents in comparison to the flanking regions. A low translational rate and weak relationship between RSCU and tRNA were obtained which may be due to the absence of optimal codons.
Conclusion: In this study, we have uncovered the human miRNAs that have potential for binding to the coding sequences of 14 most overexpressed genes in prostate cancer and thereby could silence those genes.
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