AI Article Synopsis
- PHR1 (PHOSPHATE STARVATION RESPONSE1) is crucial for plants' response to inorganic phosphate (Pi) deficiency and promotes anthocyanin production, yet its post-translational regulation is not well understood.
- This study reveals that MdPHR1 is vital for anthocyanin accumulation in apple trees under Pi deficiency by interacting with MdWRKY75, which activates the transcription of MdMYB1, leading to increased anthocyanin levels.
- The study also finds that MdSINA1 negatively affects MdPHR1 through ubiquitination-mediated degradation, while MdBIN2 phosphorylates MdPHR1 to enhance its function and mitigate degradation by MdSINA1.
Article Abstract
PHR1 (PHOSPHATE STARVATION RESPONSE1) plays key roles in the inorganic phosphate (Pi) starvation response and in Pi deficiency-induced anthocyanin biosynthesis in plants. However, the post-translational regulation of PHR1 is unclear, and the molecular basis of PHR1-mediated anthocyanin biosynthesis remains elusive. In this study, we determined that MdPHR1 was essential for Pi deficiency-induced anthocyanin accumulation in apple (Malus × domestica). MdPHR1 interacted with MdWRKY75, a positive regulator of anthocyanin biosynthesis, to enhance the MdWRKY75-activated transcription of MdMYB1, leading to anthocyanin accumulation. In addition, the E3 ubiquitin ligase SEVEN IN ABSENTIA1 (MdSINA1) negatively regulated MdPHR1-promoted anthocyanin biosynthesis via the ubiquitination-mediated degradation of MdPHR1. Moreover, the protein kinase apple BRASSINOSTEROID INSENSITIVE2 (MdBIN2) phosphorylated MdPHR1 and positively regulated MdPHR1-mediated anthocyanin accumulation by attenuating the MdSINA1-mediated ubiquitination degradation of MdPHR1. Taken together, these findings not only demonstrate the regulatory role of MdPHR1 in Pi starvation induced anthocyanin accumulation, but also provide an insight into the post-translational regulation of PHR1.
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| http://dx.doi.org/10.1111/jipb.13538 | DOI Listing |
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