AI Article Synopsis
- The study aimed to compare recombinant protein levels from three Eimeria genes in E. coli before and after codon optimization.
- The results showed varying outcomes in protein yield, indicating that codon optimization doesn't always lead to higher expression levels.
- Codon analysis revealed that Eimeria genes mix rare and common codons, but no link was found between codon choice and protein structure, suggesting other factors influence codon selection in this parasite.
Article Abstract
The objective of this study was to compare the levels of recombinant protein from three Eimeria genes before and after optimization of codons for expression in Escherichia coli. Protein coding sequences from Eimeria maxima (EmaxSO7, EmaxIMP1) and Eimeria acervulina (EAH00033530) were cloned with or without prior codon optimization and expressed as polyHis fusion proteins. All three outcomes: higher, lower, or no change in the yield of amount of recombinant protein were observed suggesting that codon optimization alone for expression in E. coli does not inevitably lead to higher expression levels. Analysis of codon usage for each gene sequence revealed that, similar to other organisms, Eimeria intersperses rare and frequently used codons in protein-coding sequences. However, no relationship was observed between the predicted protein structure and the location of major and minor codons, suggesting that codon selection in this apicomplexan parasite is influenced by factors other than regional secondary protein structure.
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| http://dx.doi.org/10.1016/j.mimet.2023.106750 | DOI Listing |
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