AI Article Synopsis
- Infection with Helicobacter pylori is linked to gastric cancer and biofilms help protect it from antibiotic treatment, reducing therapy effectiveness.
- A study involving 56 strains from Bangladeshi patients classified them as 19.6% high-biofilm formers and 81.4% low-biofilm formers, with no specific clade associations found.
- Significant SNPs in several genes, particularly alpB, were associated with high biofilm formation, indicating their potential role in detecting mutations related to biofilm development.
Article Abstract
Background: Infection with Helicobacter pylori as the cause of gastric cancer is a global public health concern. In addition to protecting germs from antibiotics, biofilms reduce the efficacy of H. pylori eradication therapy. The nucleotide polymorphisms (SNPs) related with the biofilm forming phenotype of Helicobacter pylori were studied.
Results: Fifty-six H. pylori isolate from Bangladeshi patients were included in this cross-sectional study. Crystal violet assay was used to quantify biofilm amount, and the strains were classified into high- and low-biofilm formers As a result, strains were classified as 19.6% high- and 81.4% low-biofilm formers. These phenotypes were not related to specific clades in the phylogenetic analysis. The accessories genes associated with biofilm from whole-genome sequences were extracted and analysed, and SNPs among the previously reported biofilm-related genes were analysed. Biofilm formation was significantly associated with SNPs of alpA, alpB, cagE, cgt, csd4, csd5, futB, gluP, homD, and murF (P < 0.05). Among the SNPs reported in alpB, strains encoding the N156K, G160S, and A223V mutations were high-biofilm formers.
Conclusions: This study revealed the potential role of SNPs in biofilm formation and proposed a method to detect mutation in biofilm from whole-genome sequences.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10234030 | PMC |
| http://dx.doi.org/10.1186/s12866-023-02889-8 | DOI Listing |
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