Photoelectrochemistry of a photosystem I - Ferredoxin construct on ITO electrodes.

Bioelectrochemistry

Biosystems Technology, Institute of Life Sciences and Biomedical Technologies, Technical University of Applied Sciences Wildau, 15745 Wildau, Germany. Electronic address:

Published: October 2023

In this study, photobioelectrodes based on a ferredoxin-modified photosystem I (PSI-Fd) from Thermosynechococcus vestitus have been prepared and characterized regarding the direct electron transfer between PSI-Fd and the electrode. The modified PSI with the covalently linked ferredoxin (Fd) on its stromal side has been immobilized on indium-tin-oxide (ITO) electrodes with a 3-dimensional inverse-opal structure. Compared to native PSI, a lower photocurrent and a lower onset potential of the cathodic photocurrent have been observed. This can be mainly attributed to a different adsorption behavior of the PSI-Fd-construct onto the 3D ITO. However, the overall behavior is rather similar to PSI. First experiments have been performed for applying this PSI-Fd photobioelectrode for enzyme-driven NADPH generation. By coupling the electrode system with ferredoxin-NADP-reductase (FNR), first hints for the usage of photoelectrons for biosynthesis have been collected by verifying NADPH generation.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bioelechem.2023.108459DOI Listing

Publication Analysis

Top Keywords

ito electrodes
8
nadph generation
8
photoelectrochemistry photosystem
4
photosystem ferredoxin
4
ferredoxin construct
4
construct ito
4
electrodes study
4
study photobioelectrodes
4
photobioelectrodes based
4
based ferredoxin-modified
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!