AI Article Synopsis
- This study investigates the role of single-nucleotide polymorphisms (SNPs) in the NCF4 gene, specifically g.18174 A > G and g.18270 C > T, in relation to bovine mastitis and neutrophil function.
- The research involved 160 dairy cattle, using PCR-RFLP techniques to identify SNPs and flow cytometry to assess the respiratory burst of neutrophils.
- Results showed that the g.18174 A > G SNP decreased respiratory burst capacity but had no significant correlation with clinical mastitis cases.
Article Abstract
Nicotinamide adenine dinucleotide phosphate oxidase (NADPH oxidase), as a key factor in innate immunity, consists of several components, one of them is p40phox which is encoded by neutrophil cytosolic factor 4 (NCF4). Respiratory burst and reactive oxygen species (ROS) production are antimicrobial mechanisms associated with NADPH oxidase. This study evaluated the effects of g.18174 A > G and g.18270C > T single-nucleotide polymorphisms (SNP) in NCF4 on bovine mastitis and the respiratory burst capacity of neutrophils. SNPs of 160 dairy cattle were determined using a novel PCR-RFLP protocol by employing restriction enzymes, MboI and FokI. Also, the flow cytometry measured respiratory burst in 82 blood samples. Our results indicated that only g.18174 A > G SNP reduced the respiratory burst capacity. However, both SNPs were not significantly correlated with clinical mastitis. We concluded that g.18174 A > G decreases the function of NADPH oxidase. However, both SNPs were not significantly correlated with clinical mastitis.
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| http://dx.doi.org/10.1016/j.rvsc.2023.05.003 | DOI Listing |
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