AI Article Synopsis
- - Cotton is a crucial industrial crop for the global economy but faces challenges from various environmental and biological threats.
- - The development of biotechnological methods for cotton improvement is hindered by difficulties in genetic transformation and plant regeneration.
- - This text outlines an optimized protocol for creating stable transgenic cotton through somatic embryogenesis, facilitating research on cotton's response to stress and supporting global scientific efforts.
Article Abstract
Cotton is a significant industrial crop, playing an essential role in the global economy that suffers several setbacks due to biotic and abiotic adversities. Despite such problems, biotechnological advances in cotton are limited because of genetic transformation and regeneration limitations. Here, we present a detailed protocol optimized based on previously published papers, along with our modifications. These involve changes in concentration, co-cultivation time and temperature, hormones used for regeneration, media manipulation for embryogenic callus production, and efficient rescue of deformed embryos. Further, this protocol has been used in genetic studies on biotic and abiotic stress in cotton. This protocol assures a reproducible stable transgenic cotton development procedure via somatic embryogenesis that can be used by researchers worldwide. Nat Biotechnol (2016), DOI: 10.1038/nbt.3665.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227751 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.4677 | DOI Listing |
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