Discovery of the biological signaling roles of HS has spurred great interest in developing reliable methods for its accurate detection and quantification. As considerable variation in its levels is seen during pathological conditions such as sepsis, real-time quantification methods have relevance in diagnosis as well. Of various approaches, reaction-based probes which respond through 'off-on' fluorescence emission remain the most studied. Since the intensity of emission is related to the analyte concentration in these measurements, the presence of built-in features which provide an opportunity for internal referencing will be advantageous. In view of this, a dual mode response system that senses HS through characteristic fluorescence and Raman (SERS) signals based on a 1-pyrrol-3(2)-one scaffold was developed and is the main highlight of this report. This probe offers several advantages such as fast response (<1 min), and high selectivity and sensitivity with a detection limit of ∼7 nM. Imaging of HS in HepG2 cells, making use of the SERS signal from the thiolysis product is also demonstrated.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1039/d3ay00282a | DOI Listing |
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!