AI Article Synopsis
- - Ricin toxin A chain (RTA) is a lethal protein that disrupts ribosomes by targeting a specific adenine in rRNA, making it a significant focus of study for potential inhibitors.
- - Researchers have identified a potent inhibitor, -(pterin-7-carbonyl)glycyl-L-tyrosine, which demonstrates strong activity against RTA, but the exact mechanism of how it interacts with RTA was unclear before this study.
- - This study utilized X-ray crystallography to reveal that the inhibitor interacts with RTA through a hydrogen bond and causes conformational changes in certain amino acids that enhance its inhibitory effectiveness.
Article Abstract
Ricin toxin A chain (RTA), from , is a deadly protein that inactivates ribosomes by degrading an adenine residue at position 4324 in 28S rRNA. Recently, we have demonstrated that pterin-7-carboxamides with peptide pendants were potent RTA inhibitors. Among these, -(pterin-7-carbonyl)glycyl-L-tyrosine () is the most potent RTA inhibitor as a small organic molecule. However, despite this fascinating inhibitory activity, the mode of interaction of with RTA remains elusive. This study aimed to elucidate the factors responsible for the high RTA inhibitory activity of based on X-ray crystallographic analysis. Herein, we report the successfully resolved X-ray crystal structure of /RTA complexes, revealing that the interaction between the phenolic hydroxy group in and Asn78 of RTA through a hydrogen bonding and the conformational change of Tyr80 and Asn122 are responsible for the high RTA inhibitory activity of .
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10236969 | PMC |
| http://dx.doi.org/10.1080/14756366.2023.2219038 | DOI Listing |
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