AI Article Synopsis
- - Nutrient availability in chicken eggs influences early metabolism in embryos, with a focus on chickens selected for different muscle glycogen levels (pH variability).
- - The study analyzed allantoic fluids from embryos at various stages (days 10, 14, and 17) using 1H-NMR spectroscopy to identify metabolic differences between two chicken lines, pHu+ and pHu-.
- - Findings showed that pHu- embryos had more carbohydrates and specific metabolic intermediates, while pHu+ embryos had higher levels of certain amino acids and Krebs cycle metabolites, suggesting distinct nutritional environments as early as day 10 of development.
Article Abstract
Nutrient availability in eggs can affect early metabolic orientation in birds. In chickens divergently selected on the Pectoralis major ultimate pH, a proxy for muscle glycogen stores, characterization of the yolk and amniotic fluid revealed a different nutritional environment. The present study aimed to assess indicators of embryo metabolism in pHu lines (pHu+ and pHu-) using allantoic fluids (compartment storing nitrogenous waste products and metabolites), collected at days 10, 14 and 17 of embryogenesis and characterized by 1H-NMR spectroscopy. Analysis of metabolic profiles revealed a significant stage effect, with an enrichment in metabolites at the end of incubation, and an increase in interindividual variability during development. OPLS-DA analysis discriminated the two lines. The allantoic fluid of pHu- was richer in carbohydrates, intermediates of purine metabolism and derivatives of tryptophan-histidine metabolism, while formate, branched-chain amino acids, Krebs cycle intermediates and metabolites from different catabolic pathways were more abundant in pHu+. In conclusion, the characterization of the main nutrient sources for embryos and now allantoic fluids provided an overview of the in ovo nutritional environment of pHu lines. Moreover, this study revealed the establishment, as early as day 10 of embryo development, of specific metabolic signatures in the allantoic fluid of pHu+ and pHu- lines.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10232533 | PMC |
| http://dx.doi.org/10.1038/s41598-023-35652-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Acute phase proteins in canine and feline fetal fluids during the second half of gestation.
Vet Anim Sci
March 2025
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Fars, Iran.
The present study aimed to evaluate the acute phase proteins (serum amyloid A, alpha-1 acid glycoprotein, and C-reactive protein) profile in canine and feline fetal fluids during the second half of gestation. Seventeen pregnant domestic short-hair queens and 19 pregnant mixed-breed bitches were enrolled. Animals were divided into three groups according to their gestational age (30-39, 40-49, and 50-60 days).
View Article and Find Full Text PDFCalcitriol reduces Newcastle disease virus replication by modulating galectin 3 and pro-inflammatory cytokines.
Arch Virol
November 2024
Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati, Assam, 781039, India.
Calcitriol, or vitamin D (Vit D), is known for promoting strong bones and its ability to modulate inflammation and support the immune system. It has also been reported to be a potent antiviral agent, but the underlying mechanisms behind the mode of action are still unclear. Newcastle disease virus (NDV) belongs to the family Paramyxoviridae and causes infectious diseases in numerous avian species.
View Article and Find Full Text PDFCongenital urachal and urinary bladder defects leading to uroperitoneum in a neonatal quarter horse colt.
Can Vet J
November 2024
Pilchuck Veterinary Hospital, Snohomish, Washington, USA (Karam); Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, Massachusetts, USA (Arndt); Department of Surgical and Radiological Sciences (Magdesian) and Department of Medicine and Epidemiology (Dechant), School of Veterinary Medicine, University of California, Davis, California, USA; Equine Medical Center of Ocala, Ocala, Florida, USA (Cullen).
A newborn (5 h old) quarter horse colt was presented because of lethargy and severe abdominal distention. Uroperitoneum was suspected during initial workup, based on sonographic imaging and peritoneal fluid analysis. Definitive diagnosis was confirmed during exploratory celiotomy.
View Article and Find Full Text PDFVoltammetric-based immunosensing of Newcastle disease virus on polyethylene glycol-containing self-assembled monolayer modified gold electrode.
Anal Biochem
February 2025
Nanobiotechnology Research Group, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor Darul Ehsan, Malaysia; Laboratory of Virology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor Darul Ehsan, Malaysia; Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor Darul Ehsan, Malaysia. Electronic address:
A voltammetric immunosensor for the detection of Newcastle disease virus (NDV) has been developed by employing polyclonal antibody targeting NDV (anti-NDV) as a bioreceptor. Anti-NDV was immobilized on polyethylene glycol (PEG)-containing self-assembled monolayer (SAM) which was activated with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimidehydrochloride (EDC) and N-hydroxy succinimide (NHS) coupling on screen-printed gold electrode (SPGE). The introduction of PEG-containing SAM on the SPGE allowed the bioreceptor to covalently bound to the electrode surface whilst still providing a hydrophilic layer on the electrode which is important to greatly reduce non-specific bindings.
View Article and Find Full Text PDFFirst report on genetic characterization of egg drop syndrome 1976 virus in Egypt.
Braz J Microbiol
October 2024
Department of Virology, Faculty of Veterinary Medicine, Damanhour University, Damanhour, 22511, Egypt.
Article Synopsis
- The EDS-76 virus, first noted in Egypt in 1991, has been largely overlooked as a cause of reduced egg production and quality in poultry, prompting this study to investigate its genetic characteristics and impact on layer flocks.
- During 2022, researchers examined 5 backyard layer flocks that reported a 25-30% drop in egg production and noticed high rates of eggshell defects; EDS-76 virus DNA was found in 80% of these flocks.
- The newly identified BH-1 strain showed a 99.6% genetic similarity to the vaccine strain 127 but had 5 mutations that could affect the effectiveness of immunization strategies, underscoring the need for ongoing monitoring of
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!