AI Article Synopsis
- The study explores the potential of mesenchymal stem cell (MSC) and hepatocyte-derived exosomes combined with imipenem to combat inflammation in a mouse model of sepsis.
- Using the Cecal Ligation and Puncture (CLP) method to induce sepsis, the research assessed various treatments and their effects on blood and liver parameters, as well as T lymphocyte populations.
- Findings suggest that the combination of both types of exosomes with imipenem effectively reduces inflammation, supports T cell populations, minimizes liver damage, and enhances survival rates, highlighting a new strategy for sepsis treatment.
Article Abstract
Unlabelled: Aim Sepsis is a medical emergency with no definitive treatment. Animal experiments have confirmed the therapeutic characteristics of exosomes in reducing inflammation and tissue damage. The study investigates the effect of MSC and hepatocyte-derived exosomes along with imipenem in controlling systemic and local (liver) inflammation in a mouse model of sepsis.
Main Methods: To induce sepsis in C57BL/6 mice, the Cecal Ligation and Puncture (CLP) model was used. The mice were given various treatments, including imipenem, MSC-derived exosomes, hepatocyte-derived exosomes, and a mixture of exosomes. Blood and liver samples were collected and analyzed for cell blood count, liver enzymes, NO levels, cytokine concentrations, and bacterial presence. The percentages of TCD3 + CD4+/CD8+ and Treg in the spleen and mesenteric lymph nodes were also assessed using flow cytometry. The pathological changes were assessed in the liver, lung, and heart tissues. In addition, the cytokine content of exosomes was measured by ELISA.
Key Findings: Our results demonstrated that MSC-derived exosomes+imipenem could control systemic and local inflammation and increase the TCD4+ and Treg populations. Hepatocyte-derived exosomes+imipenem reduced inflammation in the liver and increased the TCD8+ and Treg populations. The mixture of exosomes+imipenem had the best function in reducing inflammation, maintaining all T lymphocyte populations, reducing liver damage, and ultimately increasing the survival rate.
Significance: The mixture of exosomes derived from MSCs and hepatocytes, along with imipenem, in the inflammatory phase of sepsis could be a promising therapeutic strategy in sepsis treatment.
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| http://dx.doi.org/10.1016/j.lfs.2023.121813 | DOI Listing |
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Article Synopsis
- The study explores the potential of mesenchymal stem cell (MSC) and hepatocyte-derived exosomes combined with imipenem to combat inflammation in a mouse model of sepsis.
- Using the Cecal Ligation and Puncture (CLP) method to induce sepsis, the research assessed various treatments and their effects on blood and liver parameters, as well as T lymphocyte populations.
- Findings suggest that the combination of both types of exosomes with imipenem effectively reduces inflammation, supports T cell populations, minimizes liver damage, and enhances survival rates, highlighting a new strategy for sepsis treatment.
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