Synthesis and in vivo evaluation of three fluid spray dried hybrid ciprofloxacin microparticles in Sprague Dawley rats.

The aim of this study is to prepare and characterise mucoadhesive silica-coated silver nanoparticles loaded with ciprofloxacin (S-AgNPs-CSCFX), and investigate serum biochemical, haematological, and histopathological effects in Sprague Dawley rats upon oral administration. S-AgNPs-CSCFX microparticles were prepared using three fluid nozzle spray drying and characterised by scanning electron microscopy (SEM), X-ray dispersive spectrometry (EDX), transmission electron microscopy (TEM), Fourier transform infrared (FTIR), zeta potential and particles size measurements and X-ray powder diffraction (XRPD). Adult male Sprague Dawley rats were randomly divided between six-treated groups, including blank S-AgNPs and S-AgNPs-CSCFX (LD: Low dose; MD: Median Dose; HD: High Dose) and control group. Each group was treated daily to evaluate the effect of the prepared particles on the lipid profile, serum biochemical, hormonal level, haemogram, and vital organ histopathology. The results showed successful encapsulation of silver nanoparticles which resulted in spherical-shaped S-AgNPs-CSCFX with an average size of 1-5 μm and surface charge of 25.2 ± 5.52 mv. The in-vivo results showed that different doses of blank S-AgNPs and S-AgNPs-CSCFX had no significant toxic effects on the physiological, biochemical, and haematological parameters. There were no marked histopathological alterations in the vital organs of the treated rats with blank and loaded particles.