Study of tick-borne zoonotic pathogens in questing and feeding ticks in Tenerife, Canary Islands, Spain.

Ticks are vectors of many zoonotic pathogens of clinical relevance, including and species. Since few data about these tick-borne pathogens are available in the Canary Islands, the aim of the present study was to screen their presence in questing and feeding ticks on the island of Tenerife. A total of 81 ticks was removed from six hedgehogs, and eight ticks were collected from the grass. PCR assays for tick species identification based on the LSU rRNA gene, and detection of spp. and spp. by targeting the 16s rRNA and gene, respectively, were carried out. and tick species were identified, with detected in In addition, and one unidentified species were detected. The overall prevalence of in ticks was 12.2%, and none harbored sp. This work constitutes the first identification in the Canary Islands of pathogenic and the species of unknown pathogenicity in questing ticks. The clinical relevance of the pathogens identified highlights the need for studies with increased sample size and locations, including potential hosts, as well as warning the population about the relevance of ticks as vectors.