sp. nov., a novel representative of the order isolated from temperate grassland soil.

Strain 0141_2 was isolated from a temperate grassland soil in Germany and was found to be affiliated with the order . It is most closely related to BR7-21, with 98.1 % 16S rRNA gene sequence similarity. Cells are rod-shaped, non-motile, stain Gram-positive and can have multiple vesicles in the cell surface. Polyhydroxybutyrate is accumulated within the cells. Catalase- and oxidase-positive. It is a mesophilic aerobe and grows best around neutral to slightly acidic pH in R2A medium. The major fatty acids are C 9, iso-C, C, C, C 7 and C 8. Diphosphatidylglycerol is present. The predominant respiratory quinone is MK-7(H). Meso-diaminopimelic acid is the diagnostic diamino acid in the cell-wall peptidoglycan. The G+C content of genomic DNA is 72.9 mol%. Based on the results of phenotypic, chemotaxonomic, genomic and phylogenetic analysis, we propose the novel species sp. nov. with the type strain 0141_2 (=DSM 104299=LMG 30000=CECT 9239).